The construction of an <i>Agrobacterium</i>-mediated transformation system of <i>Gynostemma pentaphyllum</i> using the phosphomannose-isomerase/mannose selection system
نویسندگان
چکیده
In this study, the transformed system mediated by Agrobacterium tumefaciens of Gynostemma pentaphyllum was constructed using phosphomannose-isomerase (PMI) gene as a marker. To investigate cefotaxime sodium salt (Cef) concentration bacteriostatic medium and appropriate mannose in selectable medium, explants stems with buds were cultured basic supplemented different Cef concentrations, respectively. After these optimized, 288 according protocol described above to verify their availability polymerase chain reaction (PCR), reverse transcription-PCR chlorophenol red. The results showed that for culture 150 mg l−1 3 g stem buds, According PCR results, transformation frequency 20.49% regeneration rate 29.16%. future, CPR assay could be auxiliary method choice it is moderately accurate, but has good maneuverability cost effective large-scale use.
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ژورنال
عنوان ژورنال: Plant Biotechnology
سال: 2023
ISSN: ['1347-6114', '1342-4580']
DOI: https://doi.org/10.5511/plantbiotechnology.23.0418a